Kekik ve mercanköşk diğer yağlara göre daha yüksek antimikrobiyal aktivite gösterirken, her iki mikroorganizmaya karşı en yüksek antimikrobiyal aktiviteyi. Pazarbasi, B., Yilmaz, M., Türkiye Kosullarinda Üretimi Yapilan Spirulina platensis’ in Çesitli Ekstraktlarinin Antimikrobiyal Aktivite Kapasitesinin Belirlenmesi. antimikrobiyal aktivite, antioksidan aktivite, fenolik madde. Özet: Bu çalışmada Türkiye’nin Doğu Akdeniz Bölgesi’nden toplanan Tornabea.

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Diffusion methods can be classified as disc diffusion and agar well antimikroibyal methods while dilution method can be classified as agar dilution and broth dilution methods. The concentration of essential oil which had the same turbidity with negative control was determined as MIC and it was corroborated by OD measurement. In this study, dillweed, cardamon, basil, thyme, cumin, origanum, fennel and ginger essential oils were studied for their antimicrobial activity against Escherichia coli and Staphylococcus aureus antimikribiyal agar well diffusion method.

antiikrobiyal However, the stereochemistry, lipophilicity and other factors affected the biological activity of these compounds which might be altered positively or negatively by slight modifications. Generally both of the tested microorganisms were sensitive to many of the essential oils.

However, MIC values of cumin, cardamon and origanum essential oils which were obtained by visual turbidity measurement differed from MIC values which were obtained by microplate assay. The maximum yield of dry and fresh herbage, yield and content of oil and thymol yield were obtained beginning of blooming stage.

Depends on the OD measurement results, for all the tested oils, the MBC values were equivalent to the MIC values, confirming their microbicidal effects.

Browsing by Subject “Antimikrobiyal Aktivite”

Gram-negative bacteria are less sensitive to the antimicrobials because of the lipopolysaccharide outer membrane of this group, which restricts diffusion of hydrophobic compounds. The MIC values found for microplate assay was always lower that those antimukrobiyal in turbidity measurement because of its sensitivity.

Thyme and origanum essential oils showed higher antimicrobial activity than the other essential oils.

MBC values of thyme and origanum essential oils against E. Microdilution method was used to determine MIC. Time of harvest had significant effect on yield of antimikrrobiyal herbage, content of oil and content of carvacrol.


There is therefore still a need for new methods of reducing or eliminating food borne pathogens. Inhibitory effect of these selected essential oils were monitored during incubation period 0; 2; 4; 6; 8; 10; 12; 18, 20; 22 and 24 h and the bacterial growth was determined by measuring optical density OD at nm.

In this method obtained Antimikroniyal values of E. MBC is defined as concentration where In broth dilution studies a number of different techniques exist the most used methods are that of optical density Antimilrobiyal turbidity measurement and the enumeration of colonies by viable count.

In spite of modern improvements, food safety is an increasingly important public health issue. There are also new concerns about chemical preservatives due to increasing occurrence of many respiratory illnesses. However, most studies investigating the action of whole essential oils amtimikrobiyal food spoilage organisms and food borne pathogens agree that, generally, essential oils are slightly more active against gram-positive than gram-negative bacteria.

Results showed that planting space had significant effect on plant diameter and very significant effect on other measured parameters except oil content, which was not significant. Cumin and cardamon showed the lowest antimicrobial effect against E.

Tests of antimicrobial activity can be classified as diffusion and dilution methods. Leakage of ions and other cell contents can then occur. To achieve this purpose the food industry has used different physical and chemical methods which diminish microbial growth or inhibit microorganisms and prevent or delay.

Browsing by Subject “Antimikrobiyal Aktivite”

A number of essential oil components has been identified as effective antibacterials, e. Preservation of food materials from spoilage, mainly by microbial activity, during production, storage and marketing is an important issue in the food industry. The aim of this study was antiikrobiyal determine the antimicrobial activity of some essential oils dillweed, cardamon, basil, thyme, cumin, origanum, fennel and ginger essential oils against Escherichia coli and Staphylococcus aureus and to determine the MIC and MBC values of the selected essential oils.

In this study the results show that gram-negative E.

On the contrary, no antibacterial activity was observed for basil, ginger and fennel essential oils against E. However, the increased demand for safe and natural food, without chemical preservatives, provokes many researchers to investigate the antimicrobial effects of natural compounds.


Essential oils can comprise more than sixty individual components. Hydrophobicity of essential oils and their components enables them to partition in the lipids of the bacterial cell membrane, disturbing the structures and rendering them more permeable. Numerous investigations have confirmed the antimicrobial action of essential oils. The main advantage of essential oils is that they can be used in any food and are generally recognized as safe GRAS.

However outcome of a test can be affected by factors such as the method used to extract the essential oil from plant material, the volume of inoculum, growth phase, culture medium used, pH of the media and incubation time and temperature.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Essential oils, known as volatile oils, are complex mixtures of aromatic and volatile constituents which are obtained by such as distillation, cold pressing and maceration.

H7 and Salmonella typhi was investigated. Dilution methods are usually used to obtain minimum inhibitory concentration MIC and minimum bactericidal concentration MBC. The principles and practice of these test are explained in the literature but it appears that no standardised test has been developed for evaluating the antibacterial activity of essential oils against food-related microorganisms.

The apparent antimicrobial efficacy of plant origin antimicrobials depends on factors such as the method of extracting essential oils from plant material,the part of a plant where the essential oil is extracted,type of solvent, volume of inoculum, growth phase, culture medium used, concentration of essential oil, temperature, oxygen and intrinsic or extrinsic properties of the food such as pH, fat, protein, NaCl, and physical structure of food.

Extensive loss of cell contents or the exit of critical molecules and ions will lead to death.